FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119071
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3758743.7145109467064716No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1154761.1411719514568086Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG85500.057.5053318
GAGCACA150800.056.71917716
AGAGCAC155150.055.60481615
ACACGAG54400.053.71135720
AGCACAC102500.052.8526117
CACACGA56300.051.96138419
CACGAGA61950.046.93739721
AAGAGCA240300.046.9143514
CACGTAG41200.043.8239721
CACACGT47550.042.46275319
GCACAAG70350.041.38634518
ACACGTA45550.039.48692320
ACGAGAT76600.038.00614522
GAGCAAG71000.033.9125316
AGCACAA85900.033.814617
CAGATCG212950.033.570046
GAGAGAT121350.032.27159516
AGAGAGA124150.031.93967215
AAGGGGG170700.031.83884467
AAAGGGG172650.031.64233666
Produced by FastQC (version 0.11.5)