FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11343350
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3131152.76033975853694No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC453510.3998025274720431Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52500.049.6603218
GAGCACA88450.044.63282816
AGAGCAC90700.044.29930515
AGCACAC67350.042.2973117
ACACGAG36150.039.59208720
CACACGA41700.034.9102219
CACGAGA42650.033.80054521
CACACGT34500.033.57408519
AAGAGCA170100.030.84498214
CACGTAG29850.030.82882121
ACACGTA36000.025.17622820
ACGAGAT63750.022.88755822
AAAGGGG94300.021.63667366
AAGGGGG95300.021.2981567
CGCCGTA180250.021.16103747
AGGGGGG102600.020.67251668
GCACAAG46500.020.62102118
CCGTATC177250.020.59220949
GCCGTAT192750.020.18813148
TCGCCGT193350.019.85409546
Produced by FastQC (version 0.11.5)