FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761402
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2066832.6629596044632144No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC311930.40189903834384566Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG50850.057.3287818
GAGCACA87450.053.54796616
AGAGCAC88900.053.3082415
AGCACAC62850.049.78146717
CACACGA35550.047.84050419
ACACGAG35650.047.01766620
CACGAGA38900.043.53699521
CACACGT28950.042.06585719
AAGAGCA142650.041.66472614
CACGTAG24350.040.66775521
CAGATCG86650.037.109236
ACGAGAT47250.035.84315522
CGAGATC47800.035.65011623
ACACGTA29550.033.98673620
GCACAAG43650.033.03175418
TAGATCG99150.029.7459076
ACGTAGA35850.027.23188622
GCAGATC40150.027.0505685
AGAGATC71600.026.59018117
CGTAGAT37050.026.25526823
Produced by FastQC (version 0.11.5)