FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11785366
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3454272.9309823725457487No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC341210.28952007090827725Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39050.045.2544318
GAGCACA70450.039.54092416
AGAGCAC73500.037.9491715
AGCACAC55450.035.5946617
ACACGAG28950.033.72342320
CACACGA30050.032.3727819
CACGAGA35750.027.40515121
CACACGT29800.027.12535519
CACGTAG25850.025.71835921
AAGAGCA142050.024.3673614
CGCCGTA119500.023.0165347
CCGTATC125350.022.05599649
GCCGTAT132400.021.83109348
TCGCCGT129450.021.16620846
CGTATCA135400.020.70312750
GTCGCCG151550.019.64849945
GGTCGCC151600.019.64218344
GAGGGTC19850.018.9120941
GAGGGTA36900.018.5406341
GTGGTCG159500.018.42705542
Produced by FastQC (version 0.11.5)