FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8914870
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2282042.5598129866167425No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC482930.5417128909339115Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39750.050.88524618
GAGCACA75750.046.06175616
AGAGCAC76850.045.76855515
AGCACAC52450.041.83551817
ACACGAG29250.040.9142520
CACACGA29350.040.41808319
CACGAGA35150.034.44298621
AAGAGCA139950.034.41278514
CACACGT26950.031.03296519
CAGATCG89900.029.8464016
CACGTAG22350.029.119621
GCACAAG42550.027.7160618
CGAGATC46700.025.99906523
AAAGGGG86600.025.5803566
ACGAGAT47950.025.321322
AAGGGGG88250.025.02234367
TAGATCG101200.024.9561546
AGGGGGG93350.024.93396468
CCGTATC158650.024.74837349
GTGACTG27050.024.650571
Produced by FastQC (version 0.11.5)