FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935563
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2774402.792393344997158No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC502980.5062420720396016Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55450.054.8414718
GAGCACA87850.050.07380716
AGAGCAC89650.049.65621615
AGCACAC68700.047.781417
ACACGAG32700.046.65602520
CACACGA34500.044.32478719
CACACGT36350.041.78011319
CACGAGA37550.041.09354821
AAGAGCA158700.035.44049514
CACGTAG30400.035.3353821
ACACGTA35850.030.45330420
ACGAGAT53400.029.22392722
CGAGATC54300.028.48179823
CAGATCG125500.027.1583866
AAGGGGG91450.026.09837267
CCGTATC171300.025.3107449
GCACAAG40350.025.15042718
CGCCGTA178900.024.66421147
AAAGGGG98200.024.34044566
CGTATCA182200.023.9501950
Produced by FastQC (version 0.11.5)