FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293404
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3377143.2808777349067424No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC642820.6244970079868623Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42050.052.01513318
AGAGCAC80950.045.74255415
GAGCACA79950.045.56895416
AGCACAC57950.041.1873217
ACACGAG32000.041.11824820
CACACGA32750.040.07053419
CACGAGA36700.035.27773721
AAGAGCA144700.033.13820314
CACACGT28100.031.50789519
CACGTAG25750.029.48815321
CCGTATC192850.027.76353849
AAGGGGG108650.027.60299967
CGCCGTA200050.027.23608447
CGTATCA200350.026.95116250
GCCGTAT214250.026.70467848
AGGGGGG121350.025.87172368
TCGCCGT212150.025.64968146
ACGAGAT51500.025.41157322
AAAGGGG117700.025.27369566
CAGATCG107350.025.2197916
Produced by FastQC (version 0.11.5)