FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231134
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3400593.323766456386946No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC374660.3661959661558533Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41500.048.5721718
AGAGCAC68000.042.97607415
GAGCACA66800.042.804416
AGCACAC52400.040.67412617
ACACGAG27250.038.52478820
CACACGA29050.036.25905619
CACACGT29550.032.80336819
CACGTAG23800.031.75649821
CACGAGA33300.031.52338421
AAGAGCA133000.027.21067614
CAGATCG97150.025.4188926
ACACGTA30200.024.21725320
CGCCGTA138250.024.09621647
CCGTATC138900.023.58198549
ACGAGAT46100.022.9982822
CGAGATC47250.022.95646923
GCCGTAT149350.022.84422548
TCGCCGT146350.022.64301546
CGTATCA150100.022.032250
TAGATCG108800.022.0210236
Produced by FastQC (version 0.11.5)