FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11479865
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6221075.419114249165822No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC706500.6154253556117603Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG49350.049.427818
GAGCACA92000.045.07967416
AGAGCAC92950.044.9603615
AGCACAC67200.040.51864617
ACACGAG35000.039.6942220
CACACGA36100.038.87296719
CACGAGA40500.034.90515521
AAGAGCA172000.032.926814
CACACGT33500.030.71235319
CACGTAG28800.029.40262621
GCACAAG55650.024.2743618
ACGAGAT60350.023.59817922
ACACGTA35600.023.59207220
CAGATCG183700.023.4909256
AAGGGGG121900.023.367567
AAAGGGG127800.022.28998466
CCGTATC263750.022.11791649
AGGGGGG141300.021.92063968
CGCCGTA272100.021.8502547
GAGAGAT102650.021.6844516
Produced by FastQC (version 0.11.5)