FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11546679
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4488053.8868751785686606No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC339900.294370355320348Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34350.046.15324818
AGAGCAC59450.037.91424615
GAGCACA60650.036.8747516
AGCACAC45700.036.22417
CACACGA27100.035.5127919
ACACGAG28700.033.1664520
CACGAGA34550.027.4476421
CACACGT25750.025.00702319
CGCCGTA122650.024.39343347
CCGTATC124350.023.24438549
GCCGTAT132850.023.07348348
TCGCCGT131200.022.5638346
CACGTAG23100.021.96543521
AAGAGCA131300.021.9396614
GGTCGCC147150.021.21150244
GTCGCCG146550.021.05957445
CGTATCA139200.020.9154850
GTGGTCG157650.019.6208442
AAGGGGG76400.019.6044667
TGGTCGC159450.019.22403543
Produced by FastQC (version 0.11.5)