FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686001
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3135163.60944006338475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC404630.465841530527109Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40500.051.15492218
AGAGCAC71050.047.6346715
GAGCACA72950.046.0088716
AGCACAC52050.043.8399617
CACACGA27100.040.67588419
ACACGAG26650.040.5743920
CACACGT28850.034.8122319
CACGAGA31550.033.60597621
AAGAGCA132200.032.85686514
CACGTAG24600.031.5784721
CAGATCG87750.031.1335376
ACACGTA27550.027.8171920
TAGATCG90750.027.2482786
CGAGATC41250.026.38181523
ACGAGAT41700.025.84567822
GCACAAG38500.025.17907718
CGCCGTA142650.024.03989447
CCGTATC143150.023.6396449
AGAGATC70800.023.52973217
GCCGTAT154100.023.02560648
Produced by FastQC (version 0.11.5)