FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10105175
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3867793.827533912079702No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC301030.297896869673212Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26950.044.0196518
GAGCACA52500.035.66348616
AGAGCAC55500.034.43055315
AGCACAC39550.032.6514717
CACACGA21700.031.1236419
ACACGAG21900.030.51907220
CACGAGA27950.025.03852321
CACACGT21900.023.8086619
AAGAGCA119200.021.4055214
CACGTAG19150.021.19579721
CGCCGTA115400.021.16565147
CCGTATC117000.020.51842149
GCCGTAT125250.020.2274348
TCGCCGT122500.019.82464646
GGTCGCC134700.019.1715844
CGTATCA128300.018.90219750
GTCGCCG135450.018.83245545
AAAGGGG70700.018.21592766
GTGGTCG142100.018.07380742
CAGATCG103150.018.0647936
Produced by FastQC (version 0.11.5)