FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8814911
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3284683.7262769868011145No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC385570.43740657166022434Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39500.050.7640618
GAGCACA73750.046.78788816
AGAGCAC75000.046.61733215
AGCACAC51550.042.293217
ACACGAG29600.040.66688520
CACACGA31900.038.0643119
CACGAGA34350.034.9407121
AAGAGCA140200.034.57637414
CACACGT25250.031.73607419
CACGTAG22050.031.26237721
CGCCGTA113500.029.28899247
CCGTATC115250.028.51156449
GCCGTAT130950.027.84430748
TCGCCGT121750.027.70669646
CAGATCG112550.027.1373046
CGTATCA126450.027.03784850
GCACAAG45300.026.65138218
ACACGTA26250.025.72777420
ACGAGAT46700.025.62528222
GTCGCCG158300.025.24335345
Produced by FastQC (version 0.11.5)