FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804690
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3583254.069706031671757No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC521710.5925364777181252Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG44700.054.4890718
GAGCACA71450.049.66729716
AGAGCAC73550.048.7255815
AGCACAC55850.046.9335717
CACACGA29650.044.4950819
ACACGAG30750.042.56173720
CACACGT29050.038.66822419
CACGAGA35100.038.3810521
CACGTAG26000.037.81779521
AAGAGCA135500.033.13994614
ACACGTA29150.032.65300820
ACGAGAT48350.027.64586422
CGAGATC51000.026.68948723
CGCCGTA166150.026.53708547
CCGTATC166150.026.26478249
GCCGTAT178600.025.66686448
GCACAAG33200.025.08687418
CGTAGAT39800.025.05662523
CAGATCG116250.024.9461146
TCGCCGT175950.024.91981546
Produced by FastQC (version 0.11.5)