FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403736
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG671360.5887193460108161TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC47150.028.8109721
CTGCTTG164600.028.37499458
TGCTTGA177350.026.84528259
ATGCCGG22650.026.57864870
GCTTGAA193500.025.68998360
ACGTGTG31300.025.1678451
CCGTCTT208750.024.30918351
GCCGTCT209200.024.1565250
TGCCGTC224900.023.83955449
ATGCCGT239800.022.6647648
CTTCTGC216600.022.43556255
GTGTGCT60200.022.3828752
CGTCTTC225250.022.18939852
TCTGCTT217000.021.82960757
TATGCCG249450.021.74712447
GTCTTCT231300.021.10963653
GATCTCG253100.020.16494640
GTATGCC274000.019.99012446
TTCTGCT234350.019.95948856
ATCTCGT260950.019.61311141
Produced by FastQC (version 0.11.5)