FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278287
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG406520.3604448086841557TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG39650.026.0502281
CGTGTGC56000.024.0091021
CTGCTTG114000.023.33866358
GCCGTCT139950.021.45666550
TGCCGTC155550.021.23976949
CCGTCTT145800.020.88361751
TGCTTGA130200.020.70163359
ATGCCGT167500.019.99599648
GTGTGCT67450.019.8735852
TATGCCG174350.019.59351247
GCTTGAA146950.019.36574760
GATCTCG169250.019.02624940
CACGATC173650.019.02572437
GACGTGT26700.019.0146981
CGTCTTC162900.018.221152
CGATCTC178150.018.17397539
ATCTCGT179450.017.86835541
ACGATCT182350.017.85175338
GTATGCC194950.017.7204446
CTCGTAT192000.017.17442543
Produced by FastQC (version 0.11.5)