FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296809
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA727151.1547912601446224TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121970.19370128584176524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT247200.046.0045661
ACGTGTG332500.034.243722
TCCGCTC37600.029.51593613
TCACAGA187950.029.26861631
CACAGAT189200.029.0204532
CGTGTGC403250.028.2877583
GTGTGCT406300.028.0323354
GTCACAG202350.027.27221930
TTCCGCT41700.026.94778412
GCACCCG18850.026.9256338
GTGCTCT426000.026.686076
CTTCCGC42850.026.30312711
CGATCTA101400.026.2745515
ACAGATC209100.026.10795833
TGTGCTC442050.025.7890245
GGGTGGC20200.024.94929570
GCGCACA15500.024.8357436
GCTCTTC463750.024.4183648
TGCTCTT468250.024.3107437
TCTTCCG471600.023.97823510
Produced by FastQC (version 0.11.5)