FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119071
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG1207961.1937459476270105TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG55750.036.8654441
CGTGTGC104400.030.6864641
GCTTGAA262950.029.07414660
CTGCTTG243400.028.59286358
TGCTTGA248700.028.44593259
AAAAGGG143700.028.05910770
GTGTGCT117200.027.4442272
GCGTGAA64650.026.53108660
CTTGAAA306250.025.64922561
ATGCCGG58400.025.59134770
CTTCTGC291650.025.29053155
TCTGCTT282550.025.07696257
CTGCGTG47800.024.168458
CCGTCTT344600.024.1005151
GCCGTCT340000.023.95319250
TTCTGCT276900.023.80591656
TGCGTGA50250.023.75461259
GTCTTCT311400.023.74986653
CGTCTTC338600.023.46492652
TGCCGTC411350.022.87826549
Produced by FastQC (version 0.11.5)