FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11343350
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC66050.025.7096611
ACGTGTG45550.025.5966571
GACGTGT27150.022.8260541
GTGTGCT77700.021.9365732
GCGGGGT48750.018.30625751
TGTGCTC97900.017.6246013
GTGCTCT102000.016.9506234
CGGGGTG61250.016.05772452
GTGTGGG33250.016.00417956
AATCGCG40900.015.91878140
GGGTGTG35400.015.72090854
CGGCGTG19400.015.696444552
GCGGCGT20100.015.32218351
TCGGGGA22700.015.26722742
TGCGGGG27750.015.00803150
AATCGGG194050.014.57531440
ATCGCGG29200.014.50625341
ATCGGGG175950.014.504106541
TGAATCG249300.014.34964538
CGGGGAT23000.014.30707543
Produced by FastQC (version 0.11.5)