FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761402
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG302000.3891049580990651TruSeq Adapter, Index 15 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG33550.031.8305381
CGTGTGC61800.028.951371
GTGTGCT69850.025.7544042
CTGCTTG70000.021.80523758
GCCGTCT85800.020.68065550
ATGCCGG28550.020.1060270
GTGCTCT93650.019.433224
GCGTGAA26200.019.37213360
TGTGCTC93350.019.3457053
GACGTGT19750.019.1466731
CCGTCTT95150.019.08958651
TGCTTGA83400.018.92932959
GCTTGAA90950.018.89685460
TGCCGTC110400.018.73523749
ATGCCGT117150.018.58184648
TATGCCG131900.017.67364747
CTTCTGC96050.016.94793155
CGTCTTC104800.016.46578252
TCTGCTT99250.015.94327157
GTATGCC152600.015.82673546
Produced by FastQC (version 0.11.5)