FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11785366
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG344660.29244743014345076TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC46850.023.0922621
ACGTGTG33600.021.5699251
GCCGTCT86950.021.53382550
TGCCGTC108150.020.35435949
TATGCCG120850.019.95441647
ATGCCGT115100.019.91586948
CCGTCTT106700.018.30226951
GTGTGCT60050.018.24262
GTATGCC137300.017.92054746
CTGCTTG86600.017.90895558
CGTATGC140500.017.1912545
TATCTCG170250.017.10593840
CTCGTAT150500.016.9565243
ATCTCGT176000.016.13065741
TCGTATG150050.016.09758244
TCTCGTA165600.015.93877242
GTCACGC218750.015.39076830
TCACGCC219950.015.33862431
TGCTTGA106450.015.32422759
CACGCCA223250.015.12763332
Produced by FastQC (version 0.11.5)