FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8914870
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG144210.16176343569788454TruSeq Adapter, Index 14 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC51800.025.2108751
ACGTGTG31350.024.457691
TATGCCG79950.023.77121247
GCCGTCT49650.023.61329350
GTGTGCT57500.022.9465142
TGCCGTC67950.022.86742249
CATCTCG60900.022.7640
GCGTGAA37500.022.58917660
ATGCCGT72600.022.46327248
GTATGCC88050.022.2601846
CGTATGC87600.022.09782445
TCGTATG82250.020.51406744
CTCGTAT62150.019.93897243
CCGTCTT64800.018.9027151
TGCGTGA31100.018.57213459
ATCTCGT72000.018.4266941
CTGCGTG27800.018.26029258
CATGTCG52900.017.9973140
TGCCGGC27650.017.97284949
TCTCGTA71000.017.4536242
Produced by FastQC (version 0.11.5)