FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935563
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG45200.029.203971
GACGTGT26050.028.7637181
GCGTGAA37000.023.46196260
TATGCCG62650.023.29611247
CGTGTGC72700.022.9732761
GCCGGCG20450.022.9323950
GTATGCC71100.021.85678146
CCGGCGT23000.021.60719351
TGCCGGC31250.021.05453549
GTGTGCT80500.020.9131682
CTGCGTG26000.020.86981658
TGCGTGA32050.020.75125159
TGCCGTC51350.020.71910549
ATGCCGT56250.020.53180148
ATGCCGG37700.020.14438848
GTGCGTG17900.019.94836858
CGTATGC76300.019.54376245
GCCGTCT36200.019.23899350
TGTGCTC98400.017.143983
CAGTGAT286850.016.9711936
Produced by FastQC (version 0.11.5)