FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293404
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG34800.027.3638081
CGTGTGC56850.025.6182671
TATCGCG51050.024.06678440
GCCGGCG15950.022.82068650
ATCGCGG35100.022.63877341
GTGTGCT67900.021.4941482
TCGGGGA29500.021.00319542
GCGGGGT48200.020.9845951
CGGGGTG59500.020.5895752
GCTTATC304550.020.54668437
CCGGCGT18800.020.10553651
CGGCGTG27850.019.73197752
TAGCTTA335350.019.71362535
GCGGCGT22750.019.69147751
TGCCGGG25950.019.69097949
CGGGGAT28800.019.69068743
ACTAGCT337800.019.62254333
GCCGGGG21050.019.6194150
ATCGGGT43000.019.61926541
CTTATCG269000.019.57022938
Produced by FastQC (version 0.11.5)