FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231134
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG38550.026.428311
CGTGTGC57150.021.9314631
GACGTGT24350.021.5671671
GTGTGCT64750.019.6750552
TATCGCG54750.019.37152340
CCGGCGT19800.018.02929351
ATCGCGG32600.017.28777341
GCCGGCG17450.017.04792250
TATGCCG34150.016.91081447
TCGCGGA17500.016.80234342
GCGTGAA30600.016.58736260
GTGCGTG15900.016.51335358
GTGCTCT78200.016.4698264
GTATCGC66550.016.46263939
GCCGGGG19700.016.34442150
ATGCCGG27750.016.2694448
TGTGCTC79200.016.2618753
TGCCGGC26000.016.1530249
CTGCGTG22400.016.09756958
CCGATGT244550.016.0857134
Produced by FastQC (version 0.11.5)