FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11479865
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG156780.13656955025167977TruSeq Adapter, Index 18 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG40000.025.733791
CGTGTGC68200.023.3584161
GCGTGAA48350.022.29919860
GTGTGCT77250.020.7957922
CATCTCG99450.020.2730340
TATGCCG77350.019.59390447
CGCATCT140500.019.50707238
CCGGCGT32300.019.17809551
TGCGTGA39800.019.0864459
GCCGGCG29150.018.969850
CTGCGTG34500.018.87551758
GTATGCC89800.018.78712546
CGTATGC83250.018.37560745
GCATCTC114900.018.3086139
CCGCATC366000.018.01469837
TGCCGGC43400.017.98288349
GCCGTCT43850.017.95796650
GTGCGTG27400.017.88885358
ATGCCGT65550.017.88612748
CATCGCG114900.017.69933940
Produced by FastQC (version 0.11.5)