FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11546679
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG361530.3131030142952792TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC43850.024.1120931
TATGCCG126900.022.45112247
TGCCGTC117850.022.03542549
GCCGTCT102850.021.94847750
ATGCCGT126200.021.2429448
CTGCTTG95050.020.699258
CCGTCTT112500.020.62567151
GTATGCC147200.019.90156746
ACGTGTG31100.019.2501141
GTGTGCT56450.018.5997962
CTCGTAT157900.018.44483643
CGTATGC158550.018.36913945
AATCTCG165950.018.22376640
ATCTCGT165150.018.01664541
TCTCGTA166650.017.70744342
TGCTTGA114650.017.55599859
TCGTATG170850.017.14928244
ACTGACC203350.017.07281733
GCTTGAA129600.016.9622960
GCGTGAA33000.016.65388560
Produced by FastQC (version 0.11.5)