FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-exoI-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-exoI-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686001
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG36400.026.740131
GACGTGT24700.023.8140051
TATGCCG39700.022.21647347
CGTGTGC56100.021.9684731
GCGTGAA25000.020.02286160
GTATGCC49000.019.49986646
GTCCATC108550.019.2475137
CATCTCG40650.019.03035240
CGTATGC46100.018.8316545
TGCCGGC20900.018.7544549
CGTCCAT188550.018.7096936
GCCGGCG14700.018.57001950
GTGTGCT67350.018.24022
ATGCCGT38750.018.15325248
CCGGCGT16000.017.93613851
CCGTCCA223650.017.60409535
TGCCGTC36250.016.99171649
CATCGCG37200.016.84322240
ACCCGTC240250.016.81018433
TCACCCG241450.016.68286731
Produced by FastQC (version 0.11.5)