FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8814911
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG651450.7390318518247093TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG28150.028.9789031
GCGTGAA36650.027.79232460
CGTGTGC51200.027.557481
CTGCTTG114000.026.84023958
GCCGTCT144650.025.54935350
GCTTGAA134800.025.1357260
TGCCGTC177000.024.77505749
CCGTCTT152900.024.74287451
TGCTTGA127600.024.47032459
ATGCCGT188000.023.99561948
TGCGTGA30950.023.41182359
GTGTGCT60800.023.0247942
TATGCCG207350.022.87251947
CATCTCG222550.022.52133640
CTTCTGC147700.022.23308255
CGTCTTC160600.022.18735952
CTGCGTG28750.021.67514258
GTATGCC225100.021.53540446
ACATCTC239700.021.27503439
ATCTCGT232800.021.20102141
Produced by FastQC (version 0.11.5)