FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804690
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG38000.032.062331
CGTGTGC62450.026.1808551
GACGTGT19850.025.5744841
GTGTGCT69850.023.6000692
CATCTCG50800.022.87573640
CATCGCG54600.022.50169440
AGGCATC177500.022.2210537
GCGTGAA31600.022.04379860
TATGCCG41300.021.4409547
CCGGCGT17450.021.2599351
GCCGGCG13650.021.02507450
GCATCTC57300.020.89150639
GTGCGTG15450.020.61913358
GGCATCT72650.020.52401738
CTGCGTG19250.020.36782858
GGCATCG120550.020.35350238
CCGGGTG16150.020.15562852
GTATGCC49000.020.0003746
ATCGCGT32700.019.91099741
ATGCCGG24100.019.89527748
Produced by FastQC (version 0.11.5)