FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119638
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204508410.696248537759972No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC678380.35480797282877424Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283070.14805196625584646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG177300.060.1725518
AGCACAC192350.055.89783517
AGAGCAC243100.055.80889515
CACACGA95550.054.80157519
ACACGAG98400.053.1502920
CACGTAG81550.052.37041521
GAGCACA243950.052.1515616
CACACGT105300.049.46143719
ACACGTA84800.046.27643620
CACGAGA119750.044.87278421
AAGAGCA349450.043.69119314
GCACGCG30800.043.63878318
CACGCGT16450.042.7683319
CGCGCGT22700.041.94065519
GCGCGCG49200.041.6893118
ACGAGAT128050.041.4992322
GTGACTG86400.041.2071
GCGCACG29200.040.5159518
TAACTGA145500.040.306682
CGTAGAT113750.039.2073123
Produced by FastQC (version 0.11.5)