FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds774-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds774-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685158
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2503462.8824576363492755No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1046051.2044110193504827Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA124000.052.75082416
AGAGCAC128450.052.0413315
ACACGAG40800.051.5541320
CACACGA42700.048.8500519
GCACACG61100.048.8022718
AGCACAC72200.046.72846217
CACGAGA45550.046.56165321
GTGACTG45850.045.2993851
GCACAAG65450.044.70316318
AAGAGCA217200.043.81305714
TGACTGG50200.042.054782
GACTGGA51650.040.6658743
GAGCAAG64200.039.6315516
AGCACAA75300.038.85553717
CACGTAG23250.036.12704521
CACAAGA82800.035.1250819
CAGATCG183300.033.7213486
ACGAGAT63300.033.5607522
AGAGATC105650.033.22561617
AGAGAGA108000.033.0217115
Produced by FastQC (version 0.11.5)