FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_ds395-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_ds395-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518942
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3274783.8441158538231623No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC710700.8342585264696014Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC100150.051.1960215
GAGCACA97750.050.9486316
GCACACG50750.048.8941818
ACACGAG35050.047.52978520
CACACGA36550.046.1534419
AGCACAC61950.044.17864617
CACGAGA40500.041.22046321
AAGAGCA170200.040.24258814
GCACAAG53000.039.55468418
GTGACTG43800.037.8218351
TGACTGG47500.035.5257452
GACTGGA49300.034.650063
ACGAGAT52700.032.34207522
CACGTAG23400.031.70804821
AGCACAA66750.031.61644217
CAGATCG125050.031.1233166
CGAGATC57650.030.2935923
CACAAGA72600.029.06866619
GAGCAAG51900.028.79419516
CTGGAGT56550.028.7799645
Produced by FastQC (version 0.11.5)