FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_Exp269-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_Exp269-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344573
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1300771.2574419456462824Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG735530.7110298317774935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC115150.045.13543715
ACACGAG38900.044.89540520
GAGCACA112900.044.88686416
CACACGA39950.044.06542219
GATCGAG38750.043.987078
GCACACG61650.043.3147818
AGATAGA140200.040.9418837
AGCACAC76700.040.01740317
GATAGAT144650.039.657778
CACGAGA45250.038.2080721
AAGAGCA192650.037.4787714
ATAGATC158050.036.2290159
GCACAAG52500.035.06477718
AGATCGA53050.033.581837
CAGATAG60000.033.07536
GCAGATC81900.032.1379745
ATCGAGA54900.031.684869
CGGGGAC8850.031.2669541
CACGTAG26550.031.24140421
TCGAGAT58700.029.69304810
Produced by FastQC (version 0.11.5)