FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_Exp269-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_Exp269-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9190862
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746500.8122197896127697No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC590420.6423989392942686Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC82650.041.0758515
GAGCACA80750.040.52380416
AGATAGA109450.037.638937
GATAGAT111900.037.064678
GATCGAG32100.036.962948
GCACACG37650.036.532218
ACACGAG27200.035.64253220
ATAGATC115000.035.4260149
AGCACAC48900.034.9266417
CACACGA27950.034.5601419
AAGAGCA155800.033.4491414
CGGGGAC8900.031.4871
CAGATAG48800.030.9124936
CACGAGA32650.030.87215621
AGATCGA41800.029.390537
GCACAAG50700.028.57857918
ATCGAGA44300.026.9412549
GAGCAAG57650.026.65055816
GCAGATC68800.026.1996445
AGAGAGA94150.026.13320715
Produced by FastQC (version 0.11.5)