FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_Exp269-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_Exp269-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786245
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1557751.5917749862179007Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1429541.4607645731330046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA61550.053.45113419
ACACGAG63550.051.65854620
GAGCACA191150.050.9932216
AGAGCAC196500.050.7984815
GATCGAG47100.050.0851978
AGATAGA184700.049.2883037
GCACACG103550.048.23201418
CACGAGA69450.047.8235921
GATAGAT192650.047.218018
AGCACAC126200.047.0634817
AAGAGCA310300.045.1284614
CAGATAG73500.042.952846
GCACAAG79850.042.4289218
ATAGATC220400.041.0503779
CACGTAG37850.040.59259821
GAGCAAG88700.039.45826316
ATCGAGA60900.038.7931189
AGCACAA89850.037.8625417
GCAGATC103350.037.6940545
ACGAGAT88950.037.37903622
Produced by FastQC (version 0.11.5)