FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS983-C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS983-C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12160069
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1873221.5404682325404568No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC482670.39693031347108304Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG16000.024.3001161
CAGATCG89200.023.306866
CGGGGAC13500.022.832541
GAGCACA46000.022.7491316
TAGATCG101950.022.58926
AGAGCAC47350.022.39655715
GATCGAG26600.021.0526078
ATAGATC87200.020.8314829
GCACACG19450.020.69338218
CGGGGAG13900.020.6635231
GATAGAT87100.020.4534788
AGATAGA94200.019.3204127
CGCCGTA235250.018.92382647
GGTCGCC241900.018.64986444
TCGCCGT239450.018.54797246
CGGGGAT21950.018.5109521
CCGTATC233350.018.49289549
GTCGCCG242850.018.49020845
GCCGTAT246350.018.32680748
CGGGGTT19700.017.78031
Produced by FastQC (version 0.11.5)