FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS983-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS983-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448353
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2558761.770970019904691No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC631110.43680411185967005Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG103500.024.0099356
TAGATCG124200.023.7281286
CGGGGAG18400.022.2705691
CGGGGTT25050.021.5315971
CGGGGAC16550.021.1624161
CGCCGTA285250.019.92529747
CGGGGTG18450.019.5525821
TCGCCGT290750.019.4762946
GGTCGCC298150.019.45096644
GCCGTAT299400.019.28746248
GTCGCCG297750.019.27713645
GCACACG25800.019.127218
CCGTATC286800.019.1099749
GAGCACA54650.018.89205716
GGGGGTA39350.018.6912271
AGATCGG444650.018.6792077
GATCGGA444750.018.6277898
GTGGTCG311500.018.5722842
AGAGCAC56050.018.4834515
CTCGGTG286250.018.34018538
Produced by FastQC (version 0.11.5)