FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS983-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS983-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296907
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1410300.8153480850651507No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC690060.3989499394313677Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA71850.029.8595316
AGAGCAC74850.029.17802415
GCACACG30600.026.30620618
GATCGAG44800.025.391518
GATAGAT146050.024.5883278
AAAGGGG135250.024.09207766
AGATAGA151300.023.7815347
ATAGATC151550.023.7418949
AAGGGGG139300.023.19022267
GGGGGAC23200.020.9862791
AGCACAC49950.020.5997717
CACACGA29000.020.51653319
CGGGGAC15100.020.4133511
TAGATCG198500.020.0135256
CAGATCG178050.019.8548976
ACACGAG31050.019.38797620
AGGGGGG173800.019.2916868
CAGATAG70950.018.7464666
AAGAGCA201350.018.59924114
AGATCGA66350.018.2524387
Produced by FastQC (version 0.11.5)