FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12519010
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2330561.861616853089821No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC408740.32649546569576987Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC13200.025.207871
TAGATCG98700.024.0421166
CAGATCG84650.023.2364356
CGGGGAG16100.021.9726261
GATCGGA338300.019.470618
AGATCGG340350.019.4152667
GGGGGTA30950.018.3333071
TCGGAAG348150.018.26610
ATCGGAA357200.018.2540049
ATAGATC81200.018.1460789
CGGGGTT23200.018.1167281
CGGGGAT25150.018.1047211
AGAGCAC44000.017.97673215
CGGGGTG19100.017.6045271
CGCCGTA220250.017.19330447
CGGAAGA371400.017.0752111
GAGGGTA39050.017.0419391
GGGGGAT28200.017.0160181
GAGCACA44850.016.9333116
GCACACG19250.016.90824318
Produced by FastQC (version 0.11.5)