FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9040878
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1120761.2396583606149756No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC444750.49193231011412825Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18300.033.27747318
AGAGCAC45250.032.87136515
GAGCACA44800.032.1857916
GATCGAG20900.030.1439428
AGATAGA80500.026.3480327
GATAGAT79500.026.1953168
AGCACAC29450.025.78824617
CACACGA15750.025.77697819
ATAGATC84150.024.8305769
GGGGACC8600.024.0173972
TAGATCG118350.023.7772466
CAGATCG101000.023.5993236
ACACGAG16900.023.40164220
GGGGGAC14100.022.3546961
AAGAGCA105850.022.3183814
CGGGGAC9900.021.2256721
CAGATAG36300.020.1517856
CACGAGA21500.019.8597421
AAAGGGG81250.019.60015166
AGATCGA34300.019.081787
Produced by FastQC (version 0.11.5)