FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_DS795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_DS795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12671932
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2191851.72968889037599No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC530900.4189574249609294Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG103000.027.8645656
TAGATCG128500.026.2300326
CGGGGAC14350.024.4098431
CGGCGCT20450.024.322711
GCACACG22550.024.0569318
AGAGCAC54150.023.78544615
CGGGGTG16950.022.9387741
GAGCACA55300.022.72074916
GATCGAG25850.022.4759488
CGGGGTT21550.022.4310051
ATAGATC100850.022.1071269
AGATAGA100050.022.0393667
GATAGAT99050.022.0142578
CGGGGAT21900.021.7526281
CGGGGAG16750.021.7488081
AGATCGG465150.020.083127
GATCGGA468300.019.9178988
GGGGGAT29050.019.6543371
GGGGGAC21000.019.515671
GGGGGTA33350.019.2208331
Produced by FastQC (version 0.11.5)