FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119638
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA773110.40435388996381627TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG616530.3224590340047233No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG597570.31254252826334894TruSeq Adapter, Index 15 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAGA336600.036.2621151
GACGTGT1218050.033.6067431
ACGTGTG1461950.028.2506352
GTCACAG204650.028.2301230
TTCAGAC450400.027.2577322
CGCGTAT108400.025.75011669
TCACAGA240950.024.8488431
GCGTATG167400.024.68450470
CGTGTGC1684600.024.6740953
TCAGACG503450.024.5934643
GTGTGCT1708600.024.323414
CACAGAT259200.023.7058532
ACGATCT242850.023.69169864
TGTCGTA166250.023.65777668
GTGCTCT1766500.023.4963236
TGTGCTC1772400.023.4241545
GTCGTAT195250.023.06747469
TCCGATC1757500.022.8607113
CCGATCT1747800.022.79528214
CATCACG480200.022.78520660
Produced by FastQC (version 0.11.5)