FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds774-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds774-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8685158
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG947081.0904579974250324TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC82400.033.708741
GCTTGAA214800.031.78918360
CTGCTTG196350.031.74529558
ACGTGTG41100.031.617581
TGCTTGA204600.031.25282759
GTGTGCT93900.029.6105422
AAAAAGG191350.029.55811770
CTTGAAA249750.027.91516561
CTTCTGC243500.026.71940455
TGAAAAA320550.026.56441163
TCTGCTT242700.026.50480357
TGCGTGA26400.026.1167259
CCGTCTT270300.025.97339651
CGTCTTC267500.025.39484652
GTCTTCT255750.025.22063853
TCTTCTG260400.024.85091254
GCCGTCT276450.024.4968850
TTGAAAA298000.024.25270562
TTCTGCT251900.024.0918356
TGCCGTC304900.024.08224349
Produced by FastQC (version 0.11.5)