FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_ds395-032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_ds395-032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518942
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG525750.6171541019999901TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG120000.032.8400958
TGCTTGA130750.030.88989659
GCTTGAA144650.029.95397860
CGTGTGC75200.029.8995911
ACGTGTG43400.029.2930771
CCGTCTT153850.028.1398651
GCCGTCT150050.027.4763550
TGCCGTC167250.027.2454949
GTGTGCT84800.026.5910872
TCTGCTT157400.026.1709257
ATGCCGT183850.025.28041548
GCGTGAA28850.025.23305560
CGTCTTC163900.025.1331752
CTTCTGC162200.025.09450555
TATGCCG203350.023.97489447
CTTGAAA189750.023.59071461
GTCTTCT166500.023.47949253
TGCGTGA25550.023.14984759
AAAAAGG170700.023.08658870
CTGCGTG23650.022.78960658
Produced by FastQC (version 0.11.5)