FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_Exp269-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_Exp269-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344573
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG651680.6299728369648511TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG382530.3697881004851529TruSeq Adapter, Index 5 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103870.10041013776015695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG245700.028.20430870
CGTGTGC86450.027.8351571
GCTTGAA288150.027.25558360
ACGTGTG48450.026.6045551
CTTGAAA309800.026.54848761
TGCTTGA279150.026.0280159
CTGCTTG275850.025.64119358
GCGTGAA58500.024.70844560
GTGTGCT100050.024.1860072
TGAAAAA447300.023.8488463
TCTGCTT329750.022.4476357
GAAAAAA487900.022.4453364
TGCGTGA51150.022.10080559
CGTGAAA70100.021.76816461
CTTCTGC342450.021.61514555
CTGCGTG49650.021.49935758
TTGAAAA405800.021.31139862
CCGTCTT380050.020.88551351
GCCGTCT360000.020.872450
TTCTGCT322750.020.8199656
Produced by FastQC (version 0.11.5)