FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_Exp269-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_Exp269-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9190862
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG446060.4853298852708266TruSeq Adapter, Index 3 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC58450.028.0421961
GTGTGCT70650.023.3921132
ACGTGTG33300.022.8226571
AAAAAGG163900.019.62451470
GCTTGAA186400.019.05791560
TGTGCTC95400.018.429181
CTTGAAA203300.018.24845561
CTGCTTG174350.018.14647558
TGCTTGA179800.017.98602559
GTGCTCT100000.017.752012
TGAAAAA287250.016.46096463
GAAAAAA295700.016.40483764
GCGTGAA35350.016.03913160
TCTGCTT217300.015.12347857
CTGCGTG30200.015.065454558
GCCGTCT229550.015.04840350
CCGTCTT243150.014.86882351
TCTTCCG202200.014.8228662
TCCGATC192150.014.796135
TGCGTGA32050.014.74207659
Produced by FastQC (version 0.11.5)