FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_Exp269-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_Exp269-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9786245
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1748951.787151251578108TruSeq Adapter, Index 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG254550.034.24964570
CGTGTGC133450.032.9126551
ACGTGTG74800.031.794511
TGAAAAA497300.030.50855463
CTTGAAA485950.030.35702561
TTGAAAA502900.029.58424262
GAAAAAA519900.029.44489164
GTGTGCT156450.028.1568892
ATGCCGG21900.026.0494770
GACGTGT39550.025.948071
GCTTGAA565650.025.78878660
AAAAAAG366500.025.1725369
TGCTTGA587750.024.43204959
CTGCTTG619950.023.01027758
TCTGCTT676150.021.30993557
CTTCTGC702200.020.61906855
TTCTGCT695150.020.59155356
TGTGCTC218250.020.215313
GTGCTCT227450.019.428324
TCTTCTG751700.019.26118954
Produced by FastQC (version 0.11.5)