FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS983-C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS983-C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12160069
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG562630.4626865192952441TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG235400.019.53603758
GCTTGAA240350.019.45420560
TGCTTGA241400.019.21010459
GCCGTCT254800.018.08965750
TGCCGTC260050.017.87249449
CCGTCTT263700.017.54548351
TATGCCG270250.017.22383747
ATGCCGT273150.017.0794148
GTATGCC279700.016.80457546
GATCTCG261400.016.68231840
CTTGAAA286650.016.65380961
CGATCAG284850.016.55010234
AGTCACG287800.016.46558629
GTCACGA289000.016.43354830
CTTCTGC288250.016.33053855
CACGATC290650.016.3162432
CGTGTGC25800.016.2912961
CGTATGC290900.016.21773345
CTCGTAT278500.016.17325243
TCTGCTT289000.016.04595457
Produced by FastQC (version 0.11.5)