FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS983-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS983-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448353
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG258060.17860859296557885TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC127800.017.38951749
TATGCCG144500.017.14786547
GTATGCC156650.017.04663846
GTCACAC362150.016.62205130
GCCGTCT110000.016.54456350
ATGCCGT135750.016.4742548
GCACACG392200.016.13392312
CGGCGTG24750.016.12051258
ACACGTC396150.016.0437314
AGTCACA378900.015.97960929
CAGTCAC379900.015.96518328
CACACGT398650.015.93428213
GAACTCC392650.015.83895322
ACGTCTG401550.015.83674516
CACGTCT403800.015.73983515
CCAGTCA385450.015.70806527
TCCAGTC386350.015.70770826
CGTATGC159400.015.566915545
AGATCGG412650.015.541161
AGCACAC407150.015.53296611
Produced by FastQC (version 0.11.5)